Essential Microbiology - Medical Course Subject
Microbiology
1. What is microbiology?
2. Why is microbiology important?
3. How do we know? Microbiology in perspective: to the �golden age� and beyond
4. Light microscopy
5. Electron microscopy
Biochemical Principles
1. Atomic structure
2. Isotopes
3. Chemical bonds
4. Acids, bases, and pH
5. Biomacromolecules
6. Carbohydrates - Biomacromolecules
7. Proteins - Biomacromolecules
8. Higher levels of protein structure
9. Nucleic acids: Structure of DNA and RNA
10. Lipids - Biomacromolecules
Cell Structure and Organisation
1. Cell Structure and Organisation
2. The procaryotic cell and its structure
3. Eucaryotic cell Structure
4. Cell division in procaryotes and eucaryotes
Microbial Nutrition and Cultivation
1. Microbial Nutrition and Cultivation
2. Microbial Nutritional categories
3. How do Microbial nutrients get into the microbial cell?
4. Laboratory cultivation of microorganisms
Microbial Growth
1. Estimation of microbial numbers
2. Factors affecting microbial growth
3. The kinetics of microbial growth
4. Growth in multicellular microorganisms
Microbial Metabolism
1. Why is energy needed?
2. Enzymes
3. Principles of energy generation
4. Oxidation - reduction reactions
5. Glycolysis
6. Aerobic respiration
7. Oxidative phosphorylation and the electron transport chain
8. Fermentation
9. Metabolism of lipids and proteins
10. Anaerobic respiration
11. Energy may be generated by the oxidation of inorganic molecules
12. Photosynthesis
13. Anoxygenic photosynthesis
14. Anabolic reactions
15. Regulation of metabolism
Microbial Diversity
1. A few words about Microbial Classification
Procaryote Diversity
1. Procaryote Diversity
2. Domain: Archaea : Classification and General features
3. Phylum: Proteobacteria
4. Photosynthetic Proteobacteria
5. Nitrifying Proteobacteria
6. Iron- and sulphur-oxidising Proteobacteria
7. Hydrogen-oxidising Proteobacteria
8. Nitrogen-fixing Proteobacteria
9. Methanotrophic Proteobacteria
10. Sulphate- and sulphur-reducing Proteobacteria
11. Enteric Proteobacteria
12. Vibrio and related genera
13. Pseudomonads
14. Acetic acid bacteria
15. Stalked and budding Proteobacteria
16. Sheathed Proteobacteria
17. Predatory Proteobacteria
18. Spirilla
19. Rickettsia
20. Phylum Cyanobacteria: the blue-green bacteria
21. Phylum Chlorobi (green sulphur bacteria) and phylum Chloroflexi (green non-sulphur bacteria)
22. Phylum Aquificae and phylum Thermotogae: the deeply branching bacteria
23. Phylum �Deinococcus-Thermus�
24. Phylum Planctomycetes
25. Phylum Chlamydiae
26. Phylum Spirochaetes
27. Phylum Bacteroidetes
28. Phylum: Verrucomicrobia
29. The Gram-positive bacteria: phylum Firmicutes and phylum Actinobacteria
30. Phylum Firmicutes: The low GC Gram-positive bacteria
31. Phylum Actinobacteria: The high GC Gram-positive bacteria
32. Bacteria and human disease
Fungi
1. General biology of the Fungi
2. Classification of the Fungi
3. Fungi and disease
Protista
1. The Algae
2. Structural characteristics of algal protists
3. Euglenophyta - Structural characteristics of algal protists
4. Dinoflagellata - Structural characteristics of algal protists
5. Diatoms - Structural characteristics of algal protists
6. Chlorophyta - Structural characteristics of algal protists
7. Phaeophyta - Structural characteristics of algal protists
8. Rhodophyta - Structural characteristics of algal protists
9. Protozoa
10. Zooflagellates (Mastigophora) - Protozoa
11. Ciliates (Ciliophora) - Protozoa
12. Amoebas (Sarcodina) - Protozoa
13. Sporozoans (Apicomplexa) - Protozoa
14. The slime moulds and water moulds (the fungus-like protists)
15. Protistan taxonomy: a modern view
Viruses
1. What are viruses?
2. Viral structure
3. Classification of viruses
4. Viral replication cycles
5. Replication cycles in bacteriophages
6. Replication cycles in animal viruses
7. Replication of RNA viruses
8. Replication cycles in plant viruses
9. Viroids
10. Prions
11. Cultivating viruses
12. Viral diseases in humans
Microbial Genetics
1. How do we know genes are made of DNA?
2. DNA replication
3. DNA replication in procaryotes
4. What happens when replication goes wrong?
5. DNA replication in eucaryotes
6. What exactly do genes do?
7. Regulation of gene expression
8. Molecular basis of mutations
9. Genetic transfer in microorganisms
Microorganisms in Genetic Engineering
1. Microorganisms in Genetic Engineering
2. Plasmid cloning vectors
3. Bacteriophages as cloning vectors
4. Expression vectors - Microorganisms in Genetic Engineering
5. Eucaryotic cloning vectors
6. Polymerase chain reaction (PCR)
The Control of Microorganisms
1. Sterilisation - Control of Microorganisms
2. Sterilisation by heat - Control of Microorganisms
3. Sterilisation by irradiation - Control of Microorganisms
4. Sterilisation using ethylene oxide - Control of Microorganisms
5. Disinfection - Control of Microorganisms
6. Kinetics of cell death
Antimicrobial Agents
1. Antimicrobial Agents
2. Antibiotics
3. What other properties should an antibiotic have?
4. How do antibiotics work?
5. Resistance to antibiotics
6. How does antibiotic resistance work?
7. How does antibiotic resistance arise?
8. Antibiotic susceptibility testing
9. Antifungal and antiviral agents
Microbial Associations
1. Microbial associations with animals
2. Microbial associations with plants
3. Microbial associations with other microorganisms
Microorganisms in the Environment
1. Microorganisms in the Environment
2. The carbon cycle - Microorganisms in the Environment
3. The nitrogen cycle - Microorganisms in the Environment
4. The sulphur cycle - Microorganisms in the Environment
5. Phosphorus - Microorganisms in the Environment
6. Microbiology of soil
7. Microbiology of freshwater
8. Microbiology of seawater
9. Beneficial effects of microorganisms in the environment
10. Harmful effects of microorganisms in the environment
Industrial and Food Microbiology
1. Microorganisms and food
2. Microorganisms as food
3. Microbial spoilage of food
4. Microorganisms in the production of biochemicals
5. Products derived from genetically engineered microorganisms
6. Microorganisms in the mining industry
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