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Basic Concept of Biotechnology - Online Course

Basic Concept of Biotechnology - Medical Course Subject



Basic Concept of Biotechnology




Biomolecules


1. Biomolecules
2. Carbohydrates
3. Classification of Carbohydrates
4. Structure of Monosaccharide�s
5. Structure of Di Saccharides and Polysaccharides
6. Importance of carbohydrates
7. Rare sugars
8. Nucleic Acids
9. Structure of Nucleic Acids
10. Watson and Crick�s double helix structure of DNA
11. Tautomerism
12. Nucleosides and nucleotides
13. Proteins - Biomolecules
14. Amino Acids and Classification of Amino Acids
15. Non-proteinogenic amino acids and its role
16. Classification of Proteins
17. Structure of Proteins
18. Biological Importance of Proteins
19. Lipids and Classification of Lipids
20. Structure of lipids
21. Fatty Acids
22. Triacylglycerols
23. Phospholipids
24. Sterols and sterol esters



Computer Applications and Biostatistics


1. Computer Applications and Biostatistics
2. Information and computer technology
3. Biostatistics
4. Types of Data - Biostatistics
5. Measures of Central Tendencies - Biostatistics
6. Types of Distribution - Biostatistics
7. Applications of Standard Error of Mean
8. Confidence Interval (CI)
9. Null Hypothesis
10. Level of Significance
11. Outliers
12. One tailed and two tailed Test
13. Importance of Sample Size Determination
14. Factors Influencing Sample Size Include
15. Sample Size Determination and Variance Estimate
16. How to Choose an Appropriate Statistical Test
17. Non-parametric tests used for analysis of different types of data
18. Common problems faced by researcher in any trial and how to address them
19. Biological data and its diversity
20. Application of computer and statistics in biology



Macromolecules and Analytical Techniques


1. Macromolecules and Analytical Techniques
2. Macromolecules
3. Centrifugation - Analysis of biomolecules
4. Chromatography: Types, Principle, Application - Analysis of biomolecules
5. Electrophoresis - Analysis of biomolecules
6. Spectroscopy - Analysis of biomolecules
7. UV Visible spectroscopy - Analysis of biomolecules
8. Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules
9. Fluorometry - Analysis of biomolecules
10. Mass Spectrometry - Analysis of biomolecules
11. Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomolecules



Plant Molecular Farming A Promising Stratergy in Biotechnology


1. Plant Molecular Farming: A Promising Stratergy in Biotechnology
2. Plants as Expression Systems
3. Types of Biomolecules and recent examples
4. Plant made pharmaceuticals (PMPs)
5. Plant made nutritional compounds (PMNs)
6. Plant made vaccines (PMVs)
7. Plant made plastics (PMPs)
8. Plant made industrial compounds (PMIs)
9. The plant endomembrane system
10. Subcellular Targeting of proteins in plants
11. Different plant species as platform for molecular farming
12. Plant Transformation
13. Optimization of transgene expression in host plant
14. Downstream processing of recombinant proteins
15. Use of affinity tags for purification
16. Use of non-chromatographic methods
17. Biosafety and regulatory issues



Plant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants


1. Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
2. The Power of Biotechnology
3. Strategies for Competitiveness - Biotechnology
4. Tools of Genetic Engineering in Plants
5. DNA Delivery Systems - Plant Transgenics
6. The Selection and Analysis of Transformants - Plant Transgenics
7. Modification of regulatory elements of marker genes - Plant Transgenics
8. More �Friendly� Selectable Markers: the Positive Selection Method - Plant Transgenics
9. Biotic Stress
10. Virus Resistance
11. RNAi Technology
12. Fungal Resistance
13. Bacterial Resistance
14. Nematode Resistance



Animal Biotechnology


1. Animal Biotechnology
2. Cell Culture - Animal Biotechnology
3. Criteria for Subculture - Animal Biotechnology
4. Culture Conditions and Mammalian Cell - Animal Biotechnology
5. Aseptic Techniques - Animal Biotechnology
6. Cryopreservation Medium
7. Protocol for Cryopreserving Cultured Cells
8. Protocol for Thawing Frozen Cells
9. Biological Contamination
10. Protocol for Microbial Decontamination
11. Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin
12. Cell Viability (quantitation and cytotoxicity)
13. Factors to Consider When Choosing a Cell Viability Assay
14. Cell Viability Assays that Measure ATP and Metabolic Capacity
15. Cytotoxicity Assays: Determining the Number of Live and Dead Cells
16. Trypan Blue
17. Concentrating Cells
18. Cell Proliferation - Animal Biotechnology
19. Cell Cycle - Animal Biotechnology
20. Cytotoxicity
21. Applications of cell culture
22. What are stem cells, and why are they important?
23. What are the unique properties of all stem cells?
24. Embryonic stem cells grown in the laboratory
25. Similarities and Differences between Embryonic and Adult stem cells
26. Potential uses of human stem cells and the obstacles
27. Genetic manipulation of animals (animal models in research)
28. Animal Breeding and Transgenic Animals
29. Methods of genetic manipulation in animals
30. A brief history of cloning
31. Cloning
32. Use of Animals in Research
33. Improving Agricultural Products with Transgenics
34. Genetically Engineered Insulin
35. Gene Therapy
36. Monoclonal Antibodies
37. Economic impact in developing countries - Animal Biotechnology
38. Ethical Issues(biosafety and regulatory issues) - Animal Biotechnology



Medical Biotechnology


1. Medical Biotechnology
2. History of Medical Biotechnology
3. Human Genome Project and its influence on medical biotechnology
4. Detecting Genetic Diseases
5. Biotech in the Hospital
6. Role of biotechnology in diagnostics and Therapeutics
7. Role of biotechnology in developing vaccines
8. Treatments developed with the aid of biotechnology
9. Other Applications of Biotechnology in Medical field
10. Designer Babies - Medical Biotechnology
11. New Biotech Breakthroughs about to change Medicine



Tools and Techniques in Biotechnology


1. Tools and Techniques in Biotechnology
2. Basic Tools in the Biotechnology
3. Measurement of Volume - Tools used in Biotechnology
4. Measurement of Weight - Tools used in Biotechnology
5. Measurement of pH - Tools used in Biotechnology
6. Measurement of light - Tools used in Biotechnology
7. Solution Preparation - Tools used in Biotechnology
8. Microbiological techniques - Tools used in Biotechnology
9. Preparation of biological samples for analysis - Tools used in Biotechnology
10. Separation of macromolecules - Tools used in Biotechnology
11. Manipulation of Nucleic Acids - Tools used in Biotechnology
12. Techniques of Biotechnology and Innovations
13. Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations
14. Plasmid Isolation - Techniques of Biotechnology and Innovations
15. RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations
16. Gene Isolation - Techniques of Biotechnology and Innovations
17. Molecular Mapping - Techniques of Biotechnology and Innovations
18. Genomic DNA Library Construction - Techniques of Biotechnology and Innovations
19. Transformation Techniques - Techniques of Biotechnology and Innovations
20. Direct Gene Transformation - Techniques of Biotechnology and Innovations
21. Labeling Methods - Techniques of Biotechnology and Innovations
22. Southern Blotting Technique - Techniques of Biotechnology and Innovations
23. In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations
24. Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations
25. Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations
26. DNA Sequencing Techniques - Techniques of Biotechnology and Innovations
27. Microarray - Techniques of Biotechnology and Innovations
28. Next Generation Sequencing (NGS) - Techniques of Biotechnology and Innovations



Antibiotics Microbial Sources Production and Optimization


1. Antibiotics
2. Antibacterial agents
3. Actinomycetes
4. Marine actinomycetes
5. Streptomyces
6. Identification of actinomycetes
7. Metabolite production by marine Streptomyces
8. Antagonistic marine actinomycetes in Indian peninsula
9. Optimization of fermentation conditions



Biotechnology in Forensic Sciences


1. History of Forensic Genetic Typing
2. Introduction - Biotechnology in Forensic Sciences
3. Restriction Fragment Length Polymorphism (RFLP)
4. Amplified Fragment Length Polymorphism (AMP-FLP)
5. Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences
6. Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences
7. Y-Short Tandem Repeats
8. X-chromosomal short tandem repeats (ChrX STRs)
9. mtDNA in forensic science
10. Microbial Forensics
11. Non-human DNA Forensics




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