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Biotechnology - Online Course

Biotechnology - Medical Course Subject



Biotechnology




Recombinant DNA Technology


1. Tools of rDNA technology
2. Restriction Fragment Length Polymorphism (RFLP)
3. Vectors: Vehicles for cloning
4. Plasmids
5. Vectors based on bacteriophages
6. Making rDNA
7. Introduction of rDNA into host cells - Recombinant DNA Technology
8. Identification of Recombinants - Recombinant DNA Technology
9. Polymerase Chain Reaction (PCR)
10. Hybridisation Techniques - Recombinant DNA Technology
11. DNA Library
12. DNA Sequencing
13. Site-directed Mutagenesis



Protein Structure And Engineering


1. Introduction to the World of Proteins
2. 3-D Shape of Proteins
3. Structure-Function relationship in Proteins
4. Chymotrypsin, a proteolytic enzyme
5. Molecular Disease- Sickle cell anaemia
6. Protein Finger printing- Peptide Mapping
7. 2-D Gel Electrophoresis
8. Purification of Proteins
9. Characterisation of Proteins
10. Protein Based Products
11. Designing Proteins (Protein Engineering)



Genomics and Bioinformatics


1. Genomics and Bioinformatics
2. Genome Sequencing Projects
3. Gene prediction and counting
4. Genome Similarity, SNPs and Comparative Genomics
5. Functional Genomics
6. Fluorescence in situ hybridization
7. Microarray Technology
8. Proteomics and Types of Proteomics
9. History of Bioinformatics
10. Sequences and nomenclature - Bioinformatics
11. Different types of sequences - Bioinformatics
12. Information Sources - Bioinformatics
13. Analysis using Bioinformatics tools



Microbial Cell Culture and its Applications


1. Microbial Cell Culture and its Applications
2. Microbial culture techniques
3. Measurement and kinetics of microbial growth
4. Scale-up of microbial processes
5. Isolation of microbial products
6. Strain isolation, improvement and preservation
7. Applications of microbial culture technology
8. Biosafety issues in Microbial Technology



Plant Cell Culture and Applications


1. Plant Cell Culture and Applications
2. Cell and Tissue Culture Techniques
3. Applications of Cell and Tissue Culture
4. Gene transfer methods in plants
5. Transgenic plants with beneficial traits
6. Biotic and Abiotic stress tolerance
7. Biosafety in Plant Genetic Engineering



Animal Cell Culture and Applications


1. Animal Cell Culture and Applications
2. Animal Cell Culture Techniques
3. Characterization of Cell Lines
4. Methods of Gene Delivery into Cells
5. Scale-up of Animal Culture Process
6. Applications of Animal Cell culture
7. Stem Cell Technology
8. Tissue engineering




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